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Exam 17: Biotechnology

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Question 21

Multiple Choice

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Which type of disease is LEAST likely to be able to be treated using genome editing with TALEN or CRISPR?

A) Trisomy-21
B) Leukemia
C) Cystic fibrosis
D) Huntingtons

E) B) and C)
F) A) and D)

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Question 22

Multiple Choice

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A researcher wants to create a mouse model for a disease, to allow her team to study possible therapies.The disease is known to be caused by a small deletion that removes a certain gene.Which type of mouse would be the best choice for this purpose?

A) Knockout mouse
B) Knockin mouse
C) Conditional knockout mouse activated by a drug
D) Mutant derived by traditional forward genetics screen

E) A) and C)
F) B) and D)

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Question 23

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A medical researcher is trying to develop a diagnostic technique for a disease which is characterized by replication of a particular chromosomal region.What would be the most appropriate technique?

Enzymes that cleave DNA at specific sites are called _____________.

In some ciliate protozoa, the codons UAA and UAG encode glutamate, rather than acting as STOP codons.How does this compare to other species?

Restriction enzymes are

A friend makes the argument that transgenic crops have only been used to enrich large corporations, not to improve people's health.What is the best counterexample?

Identify a sequence that is most likely to be recognized by a restriction enzyme.

Single-stranded complementary tails that are produced by restriction digestion are called ______ ends.

Single-stranded ends generated by the same restriction enzyme are complementary to each other.They can be joined together,

A 6.85 kb EcoRI fragment of DNA is shown below.The location of several restriction sites is indicated.Scale is approximate. If you were to digest this DNA with PstI, which length of DNA fragment would migrate the fastest on an agarose gel?

A commercially significant human protein now produced in bacteria is

Small circular, extrachromosomal DNA segments are known as _______.

An insecticidal protein has been discovered in a bacterium known as

You are the scientific consultant for a television show about forensic analysis.In an upcoming episode, investigators will compare the DNA of a suspect and a DNA sample collected from the victim's fingernails.You are to write up a brief explanation of the technique required for comparing the DNA.Which technique should you explain?

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another, using molecular cloning techniques?

A 6.85 kb EcoRI fragment of DNA is shown below.The location of several restriction sites is indicated.Scale is approximate. If you were to completely digest this fragment of DNA with PstI, how many pieces of DNA would you obtain?

A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme, but it also occasionally makes mistakes and cuts elsewhere.Would this type of enzyme be a useful enzyme to use in molecular cloning?

Three scientists are using different forms of PCR for their research.Abby wants to determine changes in gene expression levels in a tissue, so she uses ______.Bob is trying to extract genetic information from a wooly mammoth fossil, so he uses ______.Chris needs to express a gene in bacteria that matches the form expressed in kidney cells, so she uses ______.