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Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

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Question 21

Multiple Choice

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Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

A) Enriched
B) Reducing
C) Enumeration
D) Differential
E) Selective

F) B) and E)
G) A) and B)

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Question 22

Multiple Choice

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Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

A) the cover slip
B) the ocular
C) immersion oil
D) the condenser

E) B) and D)
F) None of the above

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Question 23

Multiple Choice

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Humans can see objects as small as 0.1 mm. Leeuwenhoek's best microscope could magnify objects to 300X. Which of the following would be invisible using Leeuwenhoek's microscope?

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following components would likely be present in a selective medium for this purpose?

A differential interference contrast microscope uses dyes to give colored, three-dimensional images.

Your microscope has a resolving power of 0.15 μm. The specimen that you will be observing has bacterial cocci the size of 0.1 μm in diameter. Which of the following statements is correct?

Which of the following parts of the microscope magnifies the specimen to produce the virtual image of the specimen?

A reducing medium is used to culture ______ organisms.

Which type of microscope cannot image live specimens?

The Gram staining procedure is best described as a ______ staining technique.

Differential media result in which of the following observable characteristics?

Which type of microscope does not use light in forming the specimen image?

Fixed smears of specimens are required to perform the Gram stain or the endospore stain.

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Which type of microscope achieves the greatest resolution and highest magnification?

The Five I's of studying microorganisms include all of the following except ______.

Agar is a complex polysaccharide that comes from a/an ______.

Which of the following is not an inoculating tool?

A textbook uses Paramecium to illustrate several types of light microscopy. Each image is magnified 230X and measures about 4 cm. The actual size of a Paramecium is about ______.

Eosin-methylene blue agar, or EMB, is a commonly used bacteriological medium for growing gram- negative bacteria from complex environments. It contains two dyes, eosin and methylene blue, as well as the sugar lactose. Eosin is a pH indicator that changes color when the medium is acidic, while methylene blue inhibits the growth of most gram-positive bacteria. If an organism consumes lactose, acid will be produced, causing the bacterial colonies to change color. based upon this description, this medium is ______.