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Discuss the ecological importance of the enzyme nitrogenase, the diversity and physiology of the various groups of organisms that contain it, and the strategies these organisms use to optimize conditions for the enzyme.

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The enzyme nitrogenase is the only enzym...

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Bacteria that contain complex internal membrane systems are more likely to divide by budding than by binary fission.

A) True
B) False

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You are looking at a sample from the top green layer of a colorful bacterial mat. Using phase contrast and fluorescence microscopy, you see evenly spaced cells in the middle of a filament that are slightly smaller than the others. The smaller cells are not fluorescent, indicating they lack photosystem II, but the rest of the filament is fluorescent. What type of filamentous bacteria are you most likely looking at?


A) purple nonsulfur bacteria
B) green sulfur bacteria
C) Acidobacteria
D) Cyanobacteria

E) All of the above
F) A) and D)

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What is convergent evolution and how is it different from horizontal gene transfer?

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Convergent evolution is the evolution of...

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Most methanotrophs are obligate ________ because ________.


A) aerobes / the initial step of methane oxidation requires O₂
B) anaerobes / methane is produced in anaerobic environments
C) fermenters / methane only provides enough energy for fermentation
D) heterotrophs / methane does not provide enough carbon for growth

E) A) and C)
F) A) and B)

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Dissimilatory nitrate reduction results in the production of gaseous products, while assimilatory nitrate reduction results in the production of ammonia for biosynthesis.

A) True
B) False

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Green sulfur bacteria are often found at the greatest depths of all phototrophic cells in lakes, oceans, and microbial mats. This is because green sulfur bacteria


A) are very sensitive to UV irradiation, despite their reliance on light for energy.
B) are the only phototrophs that can use hydrogen sulfide as an electron donor for photosynthesis.
C) are strict anaerobes.
D) have chlorosomes that are very efficient at harvesting light, allowing them to perform photosynthesis where light intensities are very low.

E) A) and D)
F) C) and D)

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What form of photosynthesis evolved first on Earth?


A) oxygenic
B) anoxygenic
C) autotrophic
D) heterotrophic

E) A) and B)
F) C) and D)

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Thiobacillus species are acidophilic chemolithotrophs capable of autotrophic growth. They perform the dissimilative oxidation of H₂S, Sᵒ, thiosulfate, or Fe⁺². Diagram or explain how Thiobacillus species obtain carbon and energy for growth and predict what type of environment they would be found in.

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Thiobacillus species obtain carbon for g...

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Dissimilatory sulfide-oxidizers use a variety of unique ecological strategies in order to


A) avoid the toxicity of hydrogen sulfide.
B) position themselves at interfaces where sulfide and oxygen meet.
C) position themselves where there is sufficient light for sulfide oxidation.
D) find organic carbon substrates.

E) None of the above
F) B) and C)

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How do Cyanobacteria impact the ecology of both aquatic and terrestrial environments on Earth?

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Cyanobacteria can contribute up to 80% o...

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How are anoxygenic phototrophic microorganisms more physiologically diverse than oxygenic phototrophic microorganisms? Be specific in your answers and use examples to illustrate your conclusions.

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Oxygenic phototrophs (Cyanobacteria) are...

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What growth conditions would you use to enrich for ammonia-oxidizing bacteria?


A) aerobic mineral media with ammonia and bicarbonate incubated in the dark
B) anaerobic mineral media with acetate and ammonia incubated in the dark
C) aerobic mineral media with acetate and ammonia incubated in the dark
D) anaerobic mineral media with ammonia and nitrate incubated in the dark

E) C) and D)
F) B) and D)

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Myxobacteria display ________ behavior and form ________ that produce myxospores.


A) gliding / slime trails
B) chemotactic / colonies
C) phototactic / slime trails
D) swarming / fruiting bodies

E) None of the above
F) A) and B)

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Dissimilative iron-reducing bacteria couple which oxidation-reduction reactions?


A) The reduction of ferric iron or manganese and the oxidation of sulfur.
B) The reduction of ferric iron or manganese and the fermentation of sugars.
C) The oxidation of H₂ or organic compounds and the reduction of ferric iron or manganese.
D) The reduction of ferric iron and photosynthesis.

E) A) and B)
F) B) and D)

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Given that multiple diverse phyla (e.g., diverse phylogenetic groups) of microorganisms can perform similar functions in ecosystems, how we might predict the microbial functions performed by a particular organism or in a particular environment without relying on growing all of the microbes in the lab?

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Without growing all of the microbes in t...

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What is one especially unique characteristic of methanotrophs?


A) They metabolize H2S.
B) They have unusually large amounts of sterols.
C) They can exist as consortia.
D) They are capable of bioluminescence.

E) A) and D)
F) All of the above

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All microbial phototrophs


A) fix carbon dioxide.
B) use water as an electron donor for photosynthesis.
C) fix carbon dioxide and use water as an electron donor for photosynthesis.
D) use light energy to drive electron transport to generate energy.

E) A) and B)
F) A) and C)

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Bioluminescence is controlled by the gene regulatory mechanism called quorum sensing, wherein the


A) depletion of oxygen is detected by an inducer molecule which activates transcription of the lux operon (genes luxCDABE) .
B) accumulation of an inducer molecule activates transcription of the lux operon (genes luxCDABE) .
C) absence of light activates an inducer molecule which activates transcription of the lux operon (genes luxCDABE) .
D) host tissues produce an autoinducer molecule which activates transcription of the lux operon (genes luxCDABE) .

E) A) and B)
F) A) and C)

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The only group of oxygenic phototrophic bacteria are the


A) green nonsulfur bacteria.
B) Chloroflexi.
C) Cyanobacteria.
D) Chlorobi.

E) C) and D)
F) B) and D)

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