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A kitchen countertop has a spill of vegetable oil that was wiped up but a film remains.What should be the next step in disinfecting this surface?


A) 10% bleach
B) spray a disinfectant that contains a heavy metal
C) warm soapy scrub
D) isopropanol
E) chlorhexidine containing solution

F) None of the above
G) A) and B)

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Which one of the following is known to be most resistant to quaternary ammonium compounds?


A) gram positive bacteria
B) members of the genus Pseudomonas
C) members of the genus Staphylococcus
D) fungi
E) enveloped viruses

F) A) and B)
G) A) and E)

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Which chemical disinfectant can be considered a sterilizing liquid agent when appropriately applied?


A) quaternary ammonium compounds
B) 70% ethanol
C) sodium benzoate
D) gluteraldehyde
E) ethylene oxide

F) A) and B)
G) D) and E)

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Application of heat to living cells can result in all of the following EXCEPT


A) breaking of hydrogen bonds.
B) alteration of membrane permeability.
C) denaturation of enzymes.
D) decreased thermal death time.
E) damage to nucleic acids.

F) A) and C)
G) B) and E)

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Figure 7.1 Figure 7.1     A suspension of 10⁶ Bacillus cereus endospores was put in a hot-air oven at 170°C.Plate counts were used to determine the number of endospores surviving at the time intervals shown. -In Figure 7.1,the decimal reduction time (D value) for the culture,which is defined as the time to reduce a population by one log,is approximately A) 0 minutes. B) 10 minutes. C) 30 minutes. D) 40 minutes. E) 60 minutes. A suspension of 10⁶ Bacillus cereus endospores was put in a hot-air oven at 170°C.Plate counts were used to determine the number of endospores surviving at the time intervals shown. -In Figure 7.1,the decimal reduction time (D value) for the culture,which is defined as the time to reduce a population by one log,is approximately


A) 0 minutes.
B) 10 minutes.
C) 30 minutes.
D) 40 minutes.
E) 60 minutes.

F) A) and B)
G) B) and D)

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Desiccation is a reliable form of sterilization.

A) True
B) False

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Which of the following treatments is the most effective for controlling microbial growth?


A) 63°C for 30 minutes
B) 72°C for 15 seconds
C) 140°C for 4 seconds
D) They are equivalent treatments.
E) None of the answers is correct.

F) A) and D)
G) None of the above

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Assume that you are responsible for decontaminating materials in a large hospital.How would you sterilize each of the following? Briefly justify your answers. a.a mattress used by a patient with bubonic plague b.intravenous glucose-saline solutions c.used disposable syringes d.tissues taken from patients

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a. To sterilize a mattress used by a pa...

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The thermal death time is the time needed to kill all the bacteria in a particular culture at a certain temperature.

A) True
B) False

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An iodophor is a(n)


A) phenol.
B) agent that reduces oxygen.
C) quaternary ammonium compound.
D) form of formaldehyde.
E) iodine mixed with a surfactant.

F) D) and E)
G) None of the above

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Which of the following regarding antimicrobial control agents is FALSE?


A) Contaminating organic debris such as blood or sputum will decrease effectiveness.
B) Some agents kill by denaturing microbial cell proteins.
C) Some agents affect microbial cell membranes by dissolving lipids.
D) Some agents are utilized as both an antiseptic and a disinfectant.
E) Most chemical agents can achieve sterility.

F) A) and B)
G) A) and C)

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Which of the following results in lethal damage to nucleic acids?


A) heat
B) radiation
C) certain chemicals
D) heat and radiation
E) heat,radiation,and some chemicals

F) A) and B)
G) B) and D)

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Which of the following pairs is mismatched?


A) Ag - wound dressings
B) biguanide - sporocide
C) CuSO₄ - algicide
D) H₂O₂ - open wounds
E) organic acids - food preservation

F) B) and C)
G) C) and D)

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All of the following substances are effective against nonenveloped viruses EXCEPT


A) alcohol.
B) chlorine.
C) ethylene oxide.
D) ozone.
E) None of the answers is correct;all of these are equally effective against nonenveloped viruses.

F) A) and B)
G) B) and C)

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If you were preparing nutrient agar at home and did not have an autoclave,what could you use to sterilize the nutrient agar?


A) bleach
B) boiling for one hour
C) hydrogen peroxide
D) oven at 121°C for one hour
E) pressure cooker at 121°C for 15 minutes

F) A) and E)
G) B) and E)

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Bone and tendons for transplant are decontaminated by


A) ethylene oxide.
B) glutaraldehyde.
C) peroxygens.
D) plasma sterilization.
E) supercritical fluids.

F) A) and E)
G) A) and D)

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Which of the following regarding antimicrobial control agents is FALSE?


A) Contaminating organic debris such as blood or sputum decrease effectiveness.
B) Some agents kill by denaturing microbial cell proteins.
C) Some agents affect microbial cell membranes by dissolving lipids.
D) Silver-impregnated dressings are used for treating antibiotic-resistant bacteria.
E) Alcohols effectively inactivate nonenveloped viruses by attacking lipids.

F) A) and C)
G) A) and B)

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Table 7.3 The following data were obtained by incubating gram-positive bacteria in nutrient medium + disinfectant for 24 hours,then transferring one loopful to nutrient medium (subculturing) .(+ = growth;- = no growth) Table 7.3 The following data were obtained by incubating gram-positive bacteria in nutrient medium + disinfectant for 24 hours,then transferring one loopful to nutrient medium (subculturing) .(+ = growth;- = no growth)     -In Table 7.3,which disinfectant was most effective against Salmonella? A) Doom B) K.O. C) Mortum D) Sterl E) The answer cannot be determined based on the information provided. -In Table 7.3,which disinfectant was most effective against Salmonella?


A) Doom
B) K.O.
C) Mortum
D) Sterl
E) The answer cannot be determined based on the information provided.

F) C) and D)
G) A) and B)

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Table 7.1 A disk-diffusion test using Staphylococcus gave the following results:  Disinfectant  Zone of inhibition (mm)  A 0 B 2.5 C 10 D 5\begin{array} { c c } \text { Disinfectant } & \text { Zone of inhibition } ( \mathrm { mm } ) \\ \text { A } & 0 \\\text { B } & 2.5 \\\text { C } & 10 \\\text { D } & 5\end{array} -In Table 7.1,which compound was the most effective against Staphylococcus?


A) A
B) B
C) C
D) D
E) The answer cannot be determined based on the information provided.

F) A) and D)
G) None of the above

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Moist heat destroys organisms by denaturing proteins.

A) True
B) False

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