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Which of the following magnifies the specimen to produce its virtual image?


A) Objective lens
B) Ocular lens
C) Condenser
D) Body
E) Iris diaphragm

F) C) and D)
G) A) and E)

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B

Which of the following is essential for development of discrete,isolated colonies?


A) Broth medium
B) Differential medium
C) Selective medium
D) Solid medium
E) Assay medium

F) A) and C)
G) B) and E)

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The relationship between a micrometer and a millimeter is ________.


A) a millimeter is 10 times larger than a micrometer
B) a micrometer is 1,000 times smaller than a millimeter
C) a millimeter is 100 times smaller than a micrometer
D) a micrometer is 1,000 times larger than a millimeter
E) a millimeter is 10 times smaller than a micrometer

F) A) and D)
G) A) and B)

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B

Bacteria that require special growth factors and complex nutrients are termed ________.


A) aerobic
B) anaerobic
C) fastidious
D) microaerophilic
E) autotrophic

F) B) and D)
G) A) and B)

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An enveloped virus measures 0.02 micrometers (μm) in diameter. What is the diameter of this virus in nanometers (nm) ?


A) 20 nm
B) 0.00002 nm
C) 2 nm
D) 0.2 nm

E) C) and D)
F) B) and D)

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A nutrient medium that has all of its chemical components identified,and their precise concentrations known and reproducible,would be termed ________.


A) a complex media
B) a reducing media
C) an enriched media
D) a chemically defined media
E) None of the choices are correct.

F) A) and B)
G) B) and D)

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A rod-shaped bacterium is measured as 0.3 micrometers (μm) in length using an ocular micrometer. Your instructor wants you to report the length in millimeters (mm) to test your understanding of metric conversions. What is the length of the organism in millimeters?


A) 0.0003 mm
B) 300 mm
C) 0.03 mm
D) 3 mm

E) All of the above
F) B) and C)

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All of the following are diameters of cells that would be resolved in a microscope with a 0.2 µm limit of resolution except ________.


A) 0.2 µm
B) 0.2 mm
C) 0.1 µm
D) 0.3 µm
E) 2.0 µm

F) All of the above
G) A) and E)

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An RN is working at a public health clinic that sees many patients with infectious disease. Ms. Hungh, a Burmese immigrant, presents to the clinic with an interpreter, complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking treatment for her condition. -Ms.Hungh's acid-fast stain results resulted as inconclusive for the presence of acid-fast bacilli.Culturing of the sputum is performed in order to isolate microbial growth for further analysis.Lowenstein-Jensen medium is utilized to select for the growth of Mycobacterium species if present in the sample,while suppressing unwanted background organisms.What is the proper term for this type of medium?  


A) Differential medium
B) Selective medium
C) Differential medium and selective medium
D) None of the choices are correct.

E) A) and C)
F) C) and D)

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Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?


A) Bright field
B) Dark field
C) Phase contrast
D) Differential interference
E) Electron

F) B) and D)
G) None of the above

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The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the ________.


A) colony
B) inoculum
C) streak
D) loop
E) incubator

F) A) and D)
G) C) and E)

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The three physical forms of laboratory media are ________.


A) solid,liquid,and gas
B) solid,semisolid,and liquid
C) streak plate,pour plate,and broth
D) aerobic,anaerobic,and micro aerobic
E) None of the choices are correct.

F) A) and E)
G) A) and D)

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At the end of the Gram stain,gram-positive bacteria will be seen as red/pink cells.

A) True
B) False

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One colony typically develops from the growth of several parent bacterial cells.

A) True
B) False

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A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?


A) The microbiologist used too much inoculum.
B) The culture is contaminated.
C) The incubation temperature was incorrect.
D) The culture medium must be selective.
E) The culture medium must be differential.

F) A) and B)
G) A) and C)

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Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?


A) Differential
B) Selective
C) Enumeration
D) Enriched
E) Reducing

F) C) and D)
G) C) and E)

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A

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?


A) Transport
B) EMB
C) Blood
D) Thioglycollate
E) General purpose

F) A) and E)
G) C) and E)

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The diameter of field for a 4x lens is measured at 4.6 mm.  How many bacterial cells,each measuring 4 μm,could be lined up along the diameter?


A) 1,150 cells
B) 1.15 cells
C) 18.4 cells
D) 115 cells
E) 1840 cells

F) All of the above
G) B) and C)

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A pure culture contains ________.


A) only one species of microorganism
B) only bacteria
C) a variety of microbes from one source
D) a variety of species from the same genus
E) None of the choices are correct.

F) C) and E)
G) C) and D)

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An RN is working at a public health clinic that sees many patients with infectious disease. Ms. Hungh, a Burmese immigrant, presents to the clinic with an interpreter, complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking treatment for her condition. -A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis.Suspecting Mycobacterium tuberculosis may be the pathogen,the RN knows the laboratory technicians will perform which stain on the sample?  


A) Endospore stain
B) Negative stain
C) Flagellar stain
D) Acid-fast stain

E) B) and D)
F) A) and D)

Correct Answer

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