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Exam 11: Labeled Immunoassays

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Question 1

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Compared to a heterogeneous assay,a homogeneous assay:

A) is more sensitive.
B) requires more procedural steps.
C) is easier to adapt to automation.
D) is more specific.

E) None of the above
F) A) and B)

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Question 2

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The standard method for detecting antinuclear antibodies (in certain autoimmune diseases) is:

A) direct immunofluorescence.
B) indirect immunofluorescence.
C) fluorescence polarization.
D) autofluorescence.

E) C) and D)
F) B) and D)

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Question 3

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Which compound would be an appropriate label in a chemiluminescent assay?

In the following equation,what is the ratio of bound radioactive (*) antigen to bound patient antigen (w:x) ??8Ag* + 4Ag + 6Ab ---> (w) Ag*Ab + (x) AgAb + (y) Ag* + (z) Ag

In which of the following testing situations is there a direct relationship between the amount of bound label and the amount of patient antigen present?

Which assay format is the best choice for measuring small molecules,such as drugs and small peptide hormones?

Indirect immunofluorescence is similar to which type of enzyme immunoassay in terms of the performance of the assay?

Compared with a capture assay,a radioimmunoassay is likely to be more advantageous in terms of:

You must choose between two widely accepted,commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration.Each kit uses a different enzyme and substrate.What is the most important consideration in making your choice?

In a capture assay to determine the presence of a viral antigen in a patient sample,which of the following would be on solid phase?

Select the technique that would most likely be used to determine the concentration of a therapeutic drug.

Which assay format has the lowest equipment cost?

An immunoassay is performed in the following manner to look for an antigen (Ag) in the patient serum: Patient serum + labeled Ag + known antibody (Ab) are added together and incubated.Unbound material is washed off.The amount of labeled Ag is then measured.What assay is performed?

An antigen is fixed to a microscope slide,then incubated with patient serum.After washing,fluorescently labeled anti-human immunoglobulin is applied.After an additional wash,the slide is inspected with a fluorescent microscope.This technique is called:

Rheumatoid factor can cause false-positive results in which type of assay?

An immunoassay is performed in the following way: Antigen is bound to a solid support.Patient serum is added,and patient antibodies bind to the antigen.The well is washed,and enzyme-labeled anti-immunoglobulin antibodies are added.The well is washed,and enzyme substrate is added.Enzyme activity is determined.Which immunoassay is described?

Identify the characteristic associated with noncompetitive enzyme immunoassays.

In the following equation,what is the ratio of free radioactive (*) antigen to free patient antigen (the ratio of y/z) after addition of the antibody and after all reactants have reached equilibrium??10 Ag* + 5 Ag + 3 Ab ---> (w) Ag*Ab + (x) AgAb + (y) Ag* + (z) Ag

Which statement about noncompetitive enzyme immunoassays is true?

Which assay poses the greatest risk of exposure to biohazards?