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_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

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Microbes can be challenging to study because they often grow in complex communities and it may be difficult to separate the various species from one another to study individually.Which of the following laboratory "tools" or techniques may be of the the most help with separating out different species from complex mixtures?


A) use of transmission electron microscopy
B) use of enriched agar for culture
C) use of selective agars for culture
D) use of light microscopy

E) All of the above
F) A) and D)

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Mannitol salt agar is selective for which bacterial genus?


A) Salmonella
B) Streptococcus
C) Neisseria
D) Staphylococcus
E) Escherichia

F) A) and E)
G) A) and D)

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Bacteria that require special growth factors and complex nutrients are termed _____.

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If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?


A) 100X
B) 950X
C) 85X
D) 850X
E) 95X

F) B) and E)
G) All of the above

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The Gram stain, acid-fast stain, and endospore stain ____________.


A) are used on a wet mount of the specimen
B) use heat to force the dye into cell structures
C) have outcomes based on cell wall differences
D) use a negative stain technique
E) are differential stains

F) B) and C)
G) B) and E)

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Compare and contrast the reagents and functions of negative staining versus positive staining.

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Answered by ExamLex AI

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Negative staining and positive staining ...

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Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?


A) fluorescence
B) differential interference contrast
C) scanning electron
D) transmission electron
E) phase-contrast

F) A) and E)
G) B) and C)

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A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.Following incubation, only the E.coli grows in the culture.What is the most likely explanation?


A) The microbiologist used too much inoculum.
B) The culture is contaminated.
C) The incubation temperature was incorrect.
D) The culture medium must be selective.
E) The culture medium must be differential

F) A) and B)
G) D) and E)

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A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of


A) negative staining.
B) using an acidic dye.
C) simple staining.
D) using the acid-fast stain.
E) capsule staining.

F) A) and D)
G) C) and D)

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The three physical forms of laboratory media are: solid, semisolid, and _____.

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A media is designed that allows only staphylococci to grow.In addition, S.aureus colonies have a yellow halo around them and other staphylococci appear white.This type of media is


A) selective only.
B) differential only.
C) both selective and differential.
D) a reducing media.
E) enriched.

F) None of the above
G) A) and E)

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A reducing medium contains


A) sugars that can be fermented.
B) extra oxygen.
C) hemoglobin, vitamins, or other growth factors.
D) substances that remove oxygen.
E) inhibiting agents.

F) None of the above
G) C) and D)

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How will E. coli appear if the mordant is not applied?


A) gram-positive
B) gram-negative
C) gram-variable
D) colorless

E) A) and D)
F) A) and C)

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Which type of media can be used to determine if a bacteria is motile?


A) SIM
B) MacConkey
C) enriched media
D) Thayer-Martin media
E) chocolate agar

F) A) and B)
G) C) and D)

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The bending of light rays as they pass from one medium to another is called refraction.

A) True
B) False

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Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?


A) resolving power
B) magnification
C) refraction
D) All of these choices are correct.

E) A) and B)
F) All of the above

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All of the following are examples of different types of microbiological media except


A) broth.
B) enriched.
C) agar.
D) Petri dish.
E) gelatin.

F) All of the above
G) B) and E)

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A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

A) True
B) False

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Which of the following media is useful for cultivating fungi?


A) Sabouraud's agar
B) MacConkey agar
C) tomato juice agar
D) phenylethanol agar
E) Mueller tellurite

F) B) and D)
G) B) and E)

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