Question 1

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Accepted Answer

A)  negative staining.
B)  using an acidic dye.
C)  simple staining.
D)  using the acid-fast stain.
E)  capsule staining.F) B) and D)
G) A) and E)

Question 2

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Accepted Answer

A) True 
 B)False  True

Question 3

_____ is the term for a culture made from one isolated colony.

Accepted Answer

The answer of _____ is the term for a culture...

Question 4

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

The answer of Magnification is achieved in a compound microscope...

Question 5

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

Accepted Answer

A)  isolation.
B)  inoculation.
C)  immunization.
D)  infection.
E)  contamination.F) A) and C)
G) A) and D)

Question 6

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Question 7

Identification relies entirely on biochemical test results.

Accepted Answer

A) True 
 B)False

Question 8

One colony typically develops from the growth of several parent bacterial cells.

Accepted Answer

A) True 
 B)False

Question 9

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Accepted Answer

The answer of _____ has the same optical qualities as...

Question 10

Which of the following media is useful for cultivating fungi?

Accepted Answer

A)  Sabouraud's agar
B)  MacConkey agar
C)  Tomato juice agar
D)  Phenylethanol agar
E)  Mueller telluriteF) C) and D)
G) C) and E) A

Question 11

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Accepted Answer

A)  streak plate
B)  spread plate
C)  pour plate
D)  All of the choices are correct
E)  None of the choices are correctF) A) and C)
G) C) and E)

Question 12

Media that contains extracts from plants, animals, or yeasts are

Accepted Answer

A)  synthetic.
B)  complex.
C)  reducing.
D)  enriched.
E)  All of the choices are correct.F) C) and D)
G) A) and D)

Question 13

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 14

Sally had a throat sample taken at a satellite lab within her health care provider's office.What kind of media would be best suited for this specimen?

Accepted Answer

A)  TSA plate
B)  TSB tube
C)  Transport media
D)  Pour plate
E)  Quadrant streak plateF) A) and B)
G) C) and D)

Question 15

Observing and characterizing colonial growth for size, shape, edge, elevation, color, odor, and texture is part of ___.

Accepted Answer

A)  Specimen collection
B)  Inoculation
C)  Incubation
D)  Isolation
E)  InspectionF) All of the above
G) C) and E)

Question 16

Which of the following is essential for development of discrete, isolated colonies?

Accepted Answer

A)  broth medium
B)  differential medium
C)  selective medium
D)  solid medium
E)  assay mediumF) D) and E)
G) C) and D)

Question 17

A reducing medium contains

Accepted Answer

A)  sugars that can be fermented.
B)  extra oxygen.
C)  hemoglobin, vitamins, or other growth factors.
D)  substances that remove oxygen.
E)  inhibiting agents.F) B) and D)
G) B) and E)

Question 18

The Six I's of studying microorganisms include all of the following except

Accepted Answer

A)  inoculation.
B)  incubation.
C)  infection.
D)  isolation.
E)  identification.F) D) and E)
G) A) and D)

Question 19

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A)  100X
B)  950X
C)  85X
D)  850X
E)  95XF) A) and E)
G) A) and B) E

Question 20

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Accepted Answer

A)  The microbiologist used too much inoculum.
B)  The culture is contaminated.
C)  The incubation temperature was incorrect.
D)  The culture medium must be selective.
E)  The culture medium must be differential.F) A) and B)
G) All of the above

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Correct AnswerverifiedShow Answer

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Correct AnswerverifiedTrue

_____ is the term for a culture made from one isolated colony.

Correct AnswerverifiedShow Answer

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct AnswerverifiedShow Answer

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

Correct AnswerverifiedShow Answer

Some microbes are not capable of growing on artificial media.

Correct AnswerverifiedShow Answer

Identification relies entirely on biochemical test results.

Correct AnswerverifiedShow Answer

One colony typically develops from the growth of several parent bacterial cells.

Correct AnswerverifiedShow Answer

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Correct AnswerverifiedShow Answer

Which of the following media is useful for cultivating fungi?

Correct AnswerverifiedA

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Correct AnswerverifiedShow Answer

Media that contains extracts from plants, animals, or yeasts are

Correct AnswerverifiedShow Answer

Mixed cultures are also referred to as contaminated cultures.

Correct AnswerverifiedShow Answer

Sally had a throat sample taken at a satellite lab within her health care provider's office.What kind of media would be best suited for this specimen?

Correct AnswerverifiedShow Answer

Observing and characterizing colonial growth for size, shape, edge, elevation, color, odor, and texture is part of ___.

Correct AnswerverifiedShow Answer

Which of the following is essential for development of discrete, isolated colonies?

Correct AnswerverifiedShow Answer

A reducing medium contains

Correct AnswerverifiedShow Answer

The Six I's of studying microorganisms include all of the following except

Correct AnswerverifiedShow Answer

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?

Correct AnswerverifiedE

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Correct AnswerverifiedShow Answer

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Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

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