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What is the function of eukaryotic RNA polymerase I?


A) Transcription of rRNA genes
B) Transcription of mRNA genes
C) Transcription of tRNA genes
D) Transcription of snRNAs
E) Initiation of transcription (but not elongation)

F) A) and B)
G) B) and E)

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Compare rho-dependent and rho-independent transcription termination in terms of their use of RNA secondary structures.

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(1)Rho-dependent transcription terminati...

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During transcription, which parts of a DNA molecule are transcribed into RNA?


A) All of the nucleotides in DNA on both strands
B) All of the nucleotides on one strand of DNA
C) Only parts of the DNA that encode mRNA
D) Only regions of the DNA that contain genes
E) Only regions of the DNA that encode rRNA

F) B) and E)
G) A) and C)

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Which of the following is found on the primary product of transcription but not on a mature mRNA molecule?


A) Start codon
B) Promoter
C) Exons
D) Introns
E) Stop codon

F) A) and E)
G) B) and D)

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In your own words, list a comprehensive definition for "gene" at the molecular level.

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A gene is a sequence of DNA, i...

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Which statement about RNA polymerase is NOT true?


A) RNA polymerase adds a ribonucleotide to the 3' end of a growing RNA molecule.
B) RNA polymerase binds to a promoter to initiate transcription.
C) During transcription of a gene, RNA polymerase reads only one strand of DNA.
D) RNA polymerase reads a template strand of DNA 5'to 3'.
E) RNA polymerase has many subunits.

F) None of the above
G) D) and E)

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In 1958, Francis Crick proposed that genes and their corresponding polypeptides are "colinear." Which of the following statements concerning the concept of colinearity is INCORRECT?


A) Colinearity means that the linear nucleotide sequence of a given gene corresponds directly to the linear amino acid sequence in the corresponding polypeptide.
B) The number of nucleotides in a gene should be precisely proportional to the number of amino acids present in the corresponding polypeptide.
C) Colinearity generally holds true for the coding regions of prokaryotic viral genes.
D) The vast majority of eukaryotic genes also follow the concept of colinearity although the size of genes may be larger.

E) A) and C)
F) A) and B)

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An in vitro transcription system that contains a bacterial gene does not initiate transcription.What is one possible problem?


A) Histones that were on the DNA when it was isolated from E.coli are blocking access to the template.
B) There is a mutation in the inverted repeat sequence that prevents a hairpin secondary structure from forming.
C) There is a mutation at -10, where a promoter consensus sequence is located.
D) Rho factor has not been added.
E) TATA-binding protein (TBP) has not been added.

F) C) and E)
G) None of the above

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In eukaryotes, which RNA polymerase transcribes the genes that encode proteins?


A) RNA polymerase I
B) RNA polymerase II
C) RNA polymerase III
D) RNA polymerase IV
E) RNA polymerase V

F) C) and E)
G) A) and B)

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If you were asked to isolate total RNA from two unknown samples and then were required to identify if the RNA was from prokaryotes or eukaryotes, what aspects regarding the classes of RNA present would help you distinguish one from the other?

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RNA from prokaryotes will cont...

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